Winnipeg Free Press (Newspaper) - April 17, 2020, Winnipeg, Manitoba
C M Y K PAGE A2
A 2 WINNIPEG FREE PRESS, FRIDAY, APRIL 17, 2020 ? WINNIPEGFREEPRESS.COM
VOL 149 NO 157
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TEST ? FROM A1 LAB ? FROM A1
BORDER ? FROM A1
PREMIER Brian Pallister took a shot
Thursday at the Winnipeg Free Press,
as he defended his plan to have some
Manitoba civil servants work reduced
hours, supplemented by Employment
Insurance benefits.
"There's a well-established work-
share program that. has been used for
some time," Pallister told a news con-
ference.
"I'm being criticized for trying to
come up with, according to one news-
paper today, some kind of a harebrained
scheme or something. If it's that bad an
idea, why are private-sector companies
like the Winnipeg Free Press using it?"
Free Press publisher Bob Cox said,
despite what the premier claimed, the
newspaper does not have any employ-
ees participating in work-share pro-
grams supplemented by EI.
Staff at the newspaper has instead
taken pay cuts ranging from 12 per
cent to 20 per cent; in the case of the
publisher, the salary reduction is 50 per
cent, Cox said.
Some travellers who make legitimate
efforts to enter the U.S. have been
turned away, he noted.
He cited a peculiarity of the way
traffic is managed by the two coun-
tries at entry points: in some cases,
Canadians who are denied entry to the
U.S. and forced to turn around have
then been ordered to quarantine by
Canadian Border Services Agency
officials.
"This is a quirk in the system that
should be looked at, since they have
not gone past the point of entry into the
United States."
The agreement is due to expire by
Tuesday, and talks to extend it have
been underway for several days, said
Deputy Prime Minister Chrystia
Freeland, who insisted that keeping
the restrictions in place would be "the
neighbourly thing to do."
Despite those "extremely friendly,
extremely neighbourly and extremely
effective" discussions, however,
Canada won't do anything that would
risk putting its citizens in jeopardy, she
insisted.
"What I can guarantee is that deci-
sions about Canada's border are taken
by Canadians, full stop," Freeland
said. "Our government will only (open
the border) when it is appropriate and
when it is not a risk to the health and
safety of Canadians."
Ontario Premier Doug Ford urged
Ottawa to "say no right away," and
said he wished Canada had closed its
doors earlier, although he was quick to
insist he wasn't criticizing the federal
government.
"Until we have this under contain-
ment, we need to keep our borders
closed," Ford said. "We should have
shut down the borders a lot sooner."
Trump issued national guidelines
Thursday aimed at steering his
country's economic recovery, even as
congressional and state lawmakers
persisted in warning of the dangers of
going too fast, too soon. The president
has eased away from his earlier insis-
tence that the decision would be his
alone to make - a power that in fact
rests with state governors.
Public health officials in the U.S.,
while they point to signs of improve-
ment in the effort to slow the rate of
infection, continue to say that main-
taining measures already in place -
physical distancing, staying home and
personal and environmental hygiene -
are critical to long-term success.
Nearly 30 per cent of the U.S. has
reported no new cases in the last week,
Trump said.
- The Canadian Press
Current testing parameters in Mani-
toba include people with symptoms
who have travelled outside the prov-
ince in the past 14 days, close contacts
of a confirmed case, health workers,
patients admitted to hospital with re-
spiratory symptoms, lab workers who
have worked with COVID-19 tests, first
responders and individuals who live or
work in the north, a remote or isolated
community, or in a congregate setting,
such as a jail.
Any person concerned about their
exposure to, or risk of having, the coro-
navirus should call Health Links to be
screened to see if a test is required.
- with files from Carol Sanders and Dylan Robertson
larry.kusch@freepress.mb.ca
Premier's jab at
paper misses mark
The rest get a matching barcode
sticker for the paperwork and the
sample.
As data-entry clerks type the req-
uisitions into a database, the labora-
tory technologists get a rack of test
tubes, which are sorted by priority.
Specimens from hospital patients
and health-care workers get tested
immediately, in individual tubes.
Meanwhile, samples from speci-
mens taken from the public and
returning travellers are combined
into a single tube - currently four
at a time. If that tube tests positive,
the remainder of those four original
specimens are tested individually.
"It allows us to tell people they're
negative faster. It's a little slower on
the positive side, but now we know
whom we need to focus on," said Van
Caeseele.
Cadham tests the samples through
a mix of semi-manual and automated
methods.
Lab staff designed the manual
method at the start of the year, based
on instructions from the National Mi-
crobiology Laboratory and on advice
from the Centers for Disease Control
and Prevention in the United States
and the World Health Organization.
The first step is called extraction.
Chemicals called reagents strip away
mucus and external parts of the
coronavirus, leaving just the "genetic
fingerprint," called RNA.
"That kind of peels away the
garbage and leaves just the virus
behind," he said. "The purer the sam-
ple, the less likely you run into the
test not working, or invalid results."
A device called a real-time ther-
mocycler creates a polymerase chain
reaction, which is when the genes
are doubled about 40 times, creating
roughly a trillion copies of the gene.
A probe sits near the sample,
coated with a reagent that creates
trace amounts of light as the specific
gene for COVID-19 is detected.
This enzymatic reaction is detect-
ed by a device called a fluorometre,
which measures the slightly yellow-
green wavelengths.
"Once we (measure) enough of that
light, then we say it's positive," said
Van Caeseele.
Much of that process has been ex-
pedited though machines called com-
mercial platform kits; Cadham uses
two machines that can handle nose
swabs and runs about 96 samples at a
time, putting the results directly into
the lab software.
Cadham gets results within 48
hours, and often on the same day.
Last month, a reagent short-
age capped testing at roughly 200
samples a day, but the lab now has
enough chemicals and machines to
process as many samples as it gets,
Van Caeseele said.
Cadham undergoes daily quality-
control tests, which include running
samples that are already known to be
positive and negative, to make sure
both the machines and the scientists
involved in the manual process give
accurate results.
About two weeks ago, National
Microbiology Laboratory staff certi-
fied Cadham's methodology, meaning
samples no longer have to be sent
around the corner to the federal lab
for confirmation.
After the testing, workers put the
swabs and vials into a device that
resembles a washing machine, which
renders the material, heating them to
the point of killing the virus.
"An enormous autoclave cooks the
baloney out of it," Van Caeseele said,
adding that the material is put in a
hazardous-waste landfill.
"COVID has put a spotlight on us,
and I have to say everybody in this
lab has stepped up and done their job
above and beyond the expectations
that I have had."
He has led the lab since 2000, but
rarely gets interview requests, which
he takes as a sign of things running
well.
"We're like the silent firefighters
of disease."
dylan.robertson@freepress.mb.ca
MIKAELA MACKENZIE / WINNIPEG FREE PRESS
Dr. Paul Van Caeseele has been in charge of the Cadham Provincial Laboratory since 2000. He and his staff have been at the forefront of Manitoba's fight against the virus.
TORONTO - Cash-strapped zoos and
aquariums in Canada are soliciting do-
nations from the public to keep their
animals fed and cared for during the
COVID-19 pandemic.
The Toronto Zoo, the Calgary Zoo and
the Vancouver Aquarium say they're
facing financial strains as they enter a
second month of closures.
The facilities typically rely on admis-
sions and parking fees to pay for food
and environment upkeep.
"It costs about $1 million a year to
feed animals at the Toronto Zoo, and
it's not like going to the pet store and
picking up a bag of 'chow' or something
like that," said Beth Gilhespy, execu-
tive director of the Toronto Zoo Wild-
life Conservancy. "They need a very
specialized diet made up of ingredients
that are natural and they would find in
the wild."
The conservancy, which is the zoo's
fundraising partner, has launched a
campaign to make up that lost revenue
so it can continue feeding and caring
for 5,000 animals, Gilhespy said.
The conservancy said it will begin
reaching out to rally supporters.
The Calgary Zoo is facing a crunch,
the chief development officer said.
Looking after its 1,000 animals costs
$550,000 per month, which includes
food and vet care, Steven Ross said.
The zoo has laid off about 60 per cent
of its staff in order to cut costs, but
without fundraising, he said, it won't be
enough to keep the animals cared for
while maintaining conservation efforts.
The Vancouver Aquarium, mean-
while, has said it could be forced into
bankruptcy and permanent closure be-
cause of the pandemic. The aquarium
has been closed since March 17 but
faces monthly costs of $1 million for
animal care and habitat maintenance.
If it doesn't find funding, aquarium
operator Ocean Wise Conservation As-
sociation said it will likely be bankrupt
by early summer.
The not-for-profit aquarium has
70,000 animals and has laid off 60 per
cent of its staff while the remainder
work reduced hours.
- The Canadian Press
Zoos raise money
to feed animals
False-positives explained
MANITOBA'S Cadham lab has had some tests come
back positive before they were confirmed as nega-
tive. Medical director Paul Van Caeseele believes
these were caused by two situations.
Trace amounts of RNA can show up so weak that
tests can't confirm whether a patient contracted
COVID-19, possibly because the patient had nearly
recovered by the time the test was taken.
Earlier on, Van Caeseele said there were a
couple of instances in which a sample came up
inconclusive.
The tube sat next to a very strong sample on the
rack, suggesting "a tiny bit of splash-over," which
can happen with some viruses.
"We know how to investigate those now, and
those false-positives don't happen anymore," he
said.
A few times, samples have shown no genetic
material (no DNA or RNA), suggesting the swab
didn't go deep enough, at which point the lab asks
the testing centre to get another sample from the
patient.
- Dylan Robertson
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